Abstract: To implement the function of marshalling station operation plan automatically, the key point was to implement the automatic optimization of needed route of marshalling station, s every operation, arrange the running route of every operation in stations and the route setting time reasonably.This paper was based on a marshalling station. By defining the insulated joints and switch points, the abstract description method of station was constructed. Punctuality rate maximum and total delay time reasonably minimum of operation as well as total route traveling length shortest were taken as optimization targets. To establish the marshalling station route choice of the multi-objective optimization model, route confilict，operation time conflict, operating planning requirements should be considered as constraint conditions. Considering 0-1 characteristics of route choice, the paper proposed Genetic Algorithm which was suitable for solving the route choice model. The actual case proved that the model could implement the two goals, avoiding mutual interference of route in time and inter space effectively, implementing the automatic execution function of marshalling station operation plan.