Abstract: In order to improve the enterprise mobile Internet application security, a security assurance system for enterprise mobile Internet applications was proposed, which was taken as safety design, development, maintenance and test basis. This paper studied on mobile Internet application penetration testing technology to learn of enterprise mobile applications easily exposed vulnerabilities, and planed the enterprise mobile Internet application security assurance system through the outline content of classified protection of information system. The assurance system completed from the viewpoint of technology safety and management safety. The mobile Internet application security assurance system constructed in this article satisfied the requirements of content integrity and platform universality, meanwhile it was laid the foundation for the formation of enterprise mobile Internet application security protection technology, detecting and judging criteria. At the end, this paper paid attention to enterprise mobile Internet application platform development and platform security problems involved. It brought an important meaning for improving the enterprise mobile application security assurance system.