Abstract: Information security was an important issue for the construction of Railway Information System. There was a lack of standardized solutions for information security level protection in railway industry. With the development of Internet, a number of service-oriented information systems on the Internet were developed, manufacturing system was connected with internet inescapability and the security threat was increased. Treble Defense-in-depth System supported by security management center was proposed in the paper which was consisted of safe compute environment, border protection and communication network protection. Combined with the characteristics of Information Systems on Internet, security program was designed in this paper. The proposed program could provide references for relevant departments.