Abstract: Based on the UIC513 standard of the International Railway Union and the characteristics of Beijing Subway, this paper proposed the calculation model and evaluation method of ride comfort of Beijing Subway, designed and developed the ride comfort management information system of Beijing Subway with ASP.NET MVC framework, Oracle database, EntityFramework+JQuery+HighCharts+Matlab and other technologies.For the validity of the system, the paper carried out a system trial run on Beijing Subway Line 1, and verified the system function by using the trial run data.The data were collected from Beijing Subway Line 1 for 9 times on Jul.26, 2018 to Oct.17, 2018, and obtained the poor and good ride comfort sections through the data analysis from the system, which was consistent with the results of passenger comfort survey in October 2018.The test results show that the design and development of ride comfort management information system of Beijing Subway is scientific and effective, which can be used in the management of ride comfort of Beijing Subway.