Abstract: Aiming at the problems existing in the current domestic computer interlocking software testing, such as low manual testing efficiency, difficult third-party testing, and heavy reliance on the technical level of testers, this paper designed and implemented a computer interlocking automatic testing system based on OpenCV image processing technology and AutoItX automatic testing library. The paper used image processing technology to acquire railway station data on the upper computer interface of computer interlocking, based on the station data, used the route search algorithm to generate the station interlocking table, and generated the relevant test cases based on the interlocking table, finally, written the test script for the test cases to implement the automatic test of the computer interlocking system. The results show that the system implements the automatic test of computer interlocking system by the third party, and can effectively reduce the manual operation, reduce the requirements for the technical level of testers, and improve the test efficiency at the same time.