Abstract: Taking the train controlled by the Guangzhou subway signal system as an example, the motion equation of the single train was constructed according to the force condition in the running process of the urban rail transit train. Based on the motion equation of the single train, the Hamilton function, the conjugate function and the complementary relaxation function were constructed. The mathematical model of the train operation process and the conditions for the establishment of the model were studied. The most energy saving control strategy in single train operation was obtained. Facing with the fastest speed train operation control strategy and the train operation control strategy of the most energy saving, the comparison experiments were carried out in the simulation environment of train operation and the Guangzhou No. 7 line respectively. The results show that the most energy saving train control strategy can reduce the energy consumption by 14.8%, significantly improve the running stability of the train.