Abstract: According to the different switching signals from corresponding different functional modules of KJ2000 train monitoring and recording devices, such as the locomotive condition signal, locomotive pressure sensor signals, a microcontroller-based analog interface circuit was developed to produce the appropriate matching signal available for offline detection of LKJ2000 train monitoring and recording device. With VS2010 development tools and MFC software-development-kits, a platform was designed on the Windows platform for human-machine interface control. To implement the two-way communication between the microcontroller analog interface circuit and the Windows platform, serial communication protocol was applied and a set of Human-machine Interface Simulation System was formed. The research results showed that the System could not only be applied to the locomotive simulator but also for the corresponding teaching and training.